Hotspot

trnK-UUU-rps16

LSC · 3937-4454 · 518 bp

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Pi 0.0154
MarkerSeek score 62.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
518
variable_sites
8
pi
0.0154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG GCTCAACCCACAAGAACTGT 50.0 50.0 58.6 58.3 641 bp 1.0 73.9
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG GTGCTCAACCCACAAGAACT 50.0 50.0 58.6 58.3 643 bp 1.0 73.9
trnK-UUU-rps16_p3 AGTCGCACTTAAAAGCCGAG TGCTCAACCCACAAGAACTG 50.0 50.0 58.6 58.3 642 bp 1.0 73.9
trnK-UUU-rps16_p4 TCGCACTTAAAAGCCGAGTA GCTCAACCCACAAGAACTGT 45.0 50.0 57.3 58.3 639 bp 1.0 72.6
trnK-UUU-rps16_p5 TCGCACTTAAAAGCCGAGTA GTGCTCAACCCACAAGAACT 45.0 50.0 57.3 58.3 641 bp 1.0 72.6