Hotspot

ycf1

SSC · 127168-133749 · 6582 bp

Back to Enkianthus

Pi 0.0228

MarkerSeek score 61.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6582
variable_sites: 409
pi: 0.0228
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCTCTTTCCGCTATGCCC	CACGGTTGATGGCGTTTATG	50.0	50.0	58.0	57.8	570-1053 bp	1.0	90.3
ycf1_p2	TTTCTCTTTCCGCTATGCCC	ACGGTTGATGGCGTTTATGT	50.0	45.0	58.0	58.2	569-1052 bp	1.0	90.2
ycf1_p3	TTTCTCTTTCCGCTATGCCC	GCAACTGCCTGTAAAGCAAC	50.0	50.0	58.0	58.2	686-1169 bp	1.0	89.9
ycf1_p4	TTTCTCTTTCCGCTATGCCC	TCCAGGAGCCAAAAATGCTT	50.0	45.0	58.0	58.0	1071-1551 bp	0.642857	76.0
ycf1_p5	TGGGTCAGTGTGTTACTCGA	TCCAGGAGCCAAAAATGCTT	50.0	45.0	58.3	58.0	1135-1615 bp	0.642857	73.5