Hotspot

ycf2-trnI-CAU

IRa · 157581-157796 · 216 bp

Back to Enkianthus

Pi 0.0222
MarkerSeek score 56.80
Primer available yes
Label ycf2-trnI-CAU

Feature Metadata

label_name
ycf2-trnI-CAU
feature_type
igs
region
IRa
length_bp
216
variable_sites
12
pi
0.0222
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnI-CAU_p1 TCCCACGAAATTGAATTGGGT TGAACCTACGAATTCGCCAA 42.9 45.0 58.1 57.5 391-473 bp 1.0 70.8
ycf2-trnI-CAU_p2 TCCCACGAAATTGAATTGGGT TTGAACCTACGAATTCGCCA 42.9 45.0 58.1 57.5 392-474 bp 1.0 70.8
ycf2-trnI-CAU_p3 TCCCACGAAATTGAATTGGGT TATTTGAGGGTCCCATTGGC 42.9 50.0 58.1 57.5 428-510 bp 1.0 70.6
ycf2-trnI-CAU_p4 CCACGAAATTGAATTGGGTCC TATTTGAGGGTCCCATTGGC 47.6 50.0 57.8 57.5 426-508 bp 1.0 69.7
ycf2-trnI-CAU_p5 CCCACGAAATTGAATTGGGTC TATTTGAGGGTCCCATTGGC 47.6 50.0 57.8 57.5 427-509 bp 1.0 69.7