Hotspot

ndhF-rpl32

SSC · 115796-116704 · 909 bp

Back to Enkianthus

Pi 0.0114

MarkerSeek score 59.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 909
variable_sites: 36
pi: 0.0114
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGAAAAAGAAGAAGGCCCA	AGCTGCCCAATATCCTTTCC	45.0	50.0	57.2	57.6	1044-1083 bp	1.0	77.1
ndhF-rpl32_p2	TGGAAAAAGAAGAAGGCCCA	GCTGCCCAATATCCTTTCCT	45.0	50.0	57.2	57.6	1043-1082 bp	1.0	77.1
ndhF-rpl32_p3	ACAAAAGGTATGATCCACGCA	GCTGCCCAATATCCTTTCCT	42.9	50.0	57.9	57.6	994-1033 bp	1.0	75.0
ndhF-rpl32_p4	TTGGAAAAAGAAGAAGGCCCA	AGCTGCCCAATATCCTTTCC	42.9	50.0	58.0	57.6	1045-1084 bp	1.0	74.9
ndhF-rpl32_p5	TTGGAAAAAGAAGAAGGCCCA	GCTGCCCAATATCCTTTCCT	42.9	50.0	58.0	57.6	1044-1083 bp	1.0	74.9