Hotspot

trnN-GUU-ndhF

IRb · 112381-113554 · 1174 bp

Back to Enkianthus

Pi 0.044

MarkerSeek score 87.90

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1174
variable_sites: 120
pi: 0.044
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GCGGGGTCGTATTTCTTCTT	50.0	50.0	58.2	58.0	1203-1476 bp	1.0	89.8
trnN-GUU-ndhF_p2	AATCAGTTAACAGCCGACCG	GCGGGGTCGTATTTCTTCTT	50.0	50.0	58.3	58.0	1184-1457 bp	1.0	88.9
trnN-GUU-ndhF_p3	CCCCAAGTAGGATTCGAACC	GCGGGGTCGTATTTCTTCTT	55.0	50.0	57.7	58.0	1211-1484 bp	1.0	88.4
trnN-GUU-ndhF_p4	CGAACCTACGACCAATCAGT	GCGGGGTCGTATTTCTTCTT	50.0	50.0	57.4	58.0	1197-1470 bp	1.0	85.9
trnN-GUU-ndhF_p5	GGATTCGAACCTACGACCAA	GCGGGGTCGTATTTCTTCTT	50.0	50.0	57.3	58.0	1202-1475 bp	1.0	85.8