Hotspot

rbcL-atpB

LSC · 57634-58860 · 1227 bp

Back to Enkianthus

Pi 0.0372
MarkerSeek score 70.80
Primer available yes
Label rbcL-atpB

Feature Metadata

label_name
rbcL-atpB
feature_type
igs
region
LSC
length_bp
1227
variable_sites
99
pi
0.0372
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.89
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-atpB_p1 GTAAGTCCATCGGTCCACAC TTTTCCTCCACGGCGATAAG 55.0 50.0 58.0 58.0 1823-2049 bp 1.0 88.3
rbcL-atpB_p2 GTAAGTCCATCGGTCCACAC CGGTTCATTCATCTGACCGT 55.0 50.0 58.0 58.0 2048-2274 bp 1.0 87.9
rbcL-atpB_p3 CGGTCTCTCCAACGCATAAA TTTTCCTCCACGGCGATAAG 50.0 50.0 58.0 58.0 2249-2475 bp 1.0 87.6
rbcL-atpB_p4 GGGGACGACCATACTTGTTC CGGTTCATTCATCTGACCGT 55.0 50.0 58.0 58.0 2329-2555 bp 1.0 87.5
rbcL-atpB_p5 CGGTCTCTCCAACGCATAAA CGGTTCATTCATCTGACCGT 50.0 50.0 58.0 58.0 2474-2700 bp 1.0 87.4