Hotspot

trnT-GGU-psbD

LSC · 33521-34988 · 1468 bp

Back to Enkianthus

Pi 0.00603

MarkerSeek score 56.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1468
variable_sites: 25
pi: 0.00603
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1617-1637 bp	1.0	78.8
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1620-1640 bp	1.0	78.6
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	55.0	50.0	58.1	57.6	1616-1636 bp	1.0	77.6
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AGAGCAATAAACCCGACCAG	55.0	50.0	58.1	57.6	1640-1660 bp	1.0	77.5
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.1	57.6	1619-1639 bp	1.0	77.4