Hotspot

petN-psbM

LSC · 30021-31241 · 1221 bp

Back to Enkianthus

Pi 0.00982

MarkerSeek score 56.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 33
pi: 0.00982
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1383-1406 bp	1.0	69.5
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1322-1345 bp	1.0	68.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1383-1406 bp	1.0	65.3
petN-psbM_p4	GGGGACAGAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.2	57.6	1405-1428 bp	1.0	64.3
petN-psbM_p5	AGGGGACAGAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.2	57.6	1406-1429 bp	1.0	64.2