Hotspot

trnK-UUU-rps16

LSC · 4607-5475 · 869 bp

Back to Enkianthus

Pi 0.00806
MarkerSeek score 55.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
869
variable_sites
22
pi
0.00806
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.94
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AGGCGGAGATTTTTAAGGAAGT 50.0 40.9 58.0 57.7 920-986 bp 1.0 67.1
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AGGCGGAGATTTTTAAGGAAGT 50.0 40.9 58.0 57.7 921-987 bp 1.0 67.1
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC AGGCGGAGATTTTTAAGGAAGT 50.0 40.9 58.0 57.7 925-991 bp 1.0 67.1
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AGGCGGAGATTTTTAAGGAAGT 50.0 40.9 58.0 57.7 923-989 bp 1.0 67.1
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGCGGAGATTTTTAAGGAAGT 50.0 40.9 58.2 57.7 871-937 bp 1.0 66.2