Hotspot

trnH-GUG-psbA

LSC · 125-831 · 707 bp

Back to Enkianthus

Pi 0.0174

MarkerSeek score 64.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 34
pi: 0.0174
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCATAACCCTGGGCGAAC	TCGTATTCCAAGCTGAGCAC	50.0	50.0	57.9	58.0	1265-1311 bp	1.0	86.0
trnH-GUG-psbA_p2	AATCATAACCCTGGGCGAAC	ATTCCAAGCTGAGCACAACA	50.0	45.0	57.9	58.0	1261-1307 bp	1.0	86.0
trnH-GUG-psbA_p3	AATCATAACCCTGGGCGAAC	TTCCAAGCTGAGCACAACAT	50.0	45.0	57.9	58.0	1260-1306 bp	1.0	86.0
trnH-GUG-psbA_p4	AATCATAACCCTGGGCGAAC	CTGATGGGATGCCTCTAGGA	50.0	55.0	57.9	58.0	1310-1356 bp	1.0	85.9
trnH-GUG-psbA_p5	AATCATAACCCTGGGCGAAC	TCTGATGGGATGCCTCTAGG	50.0	55.0	57.9	58.0	1311-1357 bp	1.0	85.9