Hotspot

ycf1

SSC · 131075-136918 · 5844 bp

Back to Engelhardia

Pi 0.00833

MarkerSeek score 36.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5844
variable_sites: 89
pi: 0.00833
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCTTTAGCCAGTTTTTCCA	TGTGCAACTTACTCATTGACAG	42.9	40.9	57.7	56.9	714-720 bp	1.0	46.8
ycf1_p2	ACCCTTTAGCCAGTTTTTCCA	TGGATTGTGCAACTTACTCA	42.9	40.0	57.7	54.9	719-725 bp	1.0	46.7
ycf1_p3	ACCCTTTAGCCAGTTTTTCCA	AGCCCGACCAAAATAAGTTATGA	42.9	39.1	57.7	58.1	585-591 bp	1.0	46.5
ycf1_p4	ACCCTTTAGCCAGTTTTTCCA	GCCCGACCAAAATAAGTTATGA	42.9	40.9	57.7	56.8	584-590 bp	1.0	45.8
ycf1_p5	ACCCTTTAGCCAGTTTTTCCA	AGCCCGACCAAAATAAGTTATG	42.9	40.9	57.7	56.8	585-591 bp	1.0	45.8