Hotspot

rpl14-rpl16

LSC · 87343-87483 · 141 bp

Back to Engelhardia

Pi 0.0317

MarkerSeek score 52.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 141
variable_sites: 13
pi: 0.0317
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TACACATCAATTCTCGGGCC	AATAGCGGCATCCAAAATGC	50.0	45.0	57.7	57.5	257-268 bp	1.0	77.2
rpl14-rpl16_p2	CCGCTGTTGTCTGCTACATT	AATAGCGGCATCCAAAATGC	50.0	45.0	58.6	57.5	237-248 bp	1.0	75.7
rpl14-rpl16_p3	CCGCTGTTGTCTGCTACAT	AATAGCGGCATCCAAAATGC	52.6	45.0	57.9	57.5	237-248 bp	1.0	71.0
rpl14-rpl16_p4	ATACACATCAATTCTCGGGCC	AATAGCGGCATCCAAAATGC	47.6	45.0	58.1	57.5	258-269 bp	1.0	70.6
rpl14-rpl16_p5	ACACATCAATTCTCGGGCC	AATAGCGGCATCCAAAATGC	52.6	45.0	57.8	57.5	256-267 bp	1.0	70.5