Hotspot

petA-psbJ

LSC · 68233-69664 · 1432 bp

Back to Engelhardia

Pi 0.0228

MarkerSeek score 59.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1432
variable_sites: 55
pi: 0.0228
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1281-1582 bp	1.0	82.9
petA-psbJ_p2	GATCCATTCCGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1299-1600 bp	1.0	81.5
petA-psbJ_p3	TGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1288-1589 bp	1.0	80.5
petA-psbJ_p4	GTGTCCAAGGCCTTTTGTTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.5	57.6	1289-1590 bp	1.0	80.1
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	1280-1581 bp	1.0	77.1