Hotspot

accD-psaI

LSC · 64009-64883 · 875 bp

Back to Engelhardia

Pi 0.0135

MarkerSeek score 61.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 24
pi: 0.0135
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTTCATGCGTTCTTTCCTC	AAAGAAGCCATCGCAATTGC	50.0	45.0	57.5	58.0	932-1155 bp	1.0	81.5
accD-psaI_p2	AAAAGGCGTTCTGAGTGAGT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.4	58.0	959-1182 bp	1.0	80.8
accD-psaI_p3	AAAGGCGTTCTGAGTGAGTT	AAAGAAGCCATCGCAATTGC	45.0	45.0	57.4	58.0	958-1181 bp	1.0	80.8
accD-psaI_p4	GCTTCATGCGTTCTTTCCTC	TCGCAATTGCCGGAAATACT	50.0	45.0	57.5	58.3	922-1145 bp	1.0	79.5
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	TCGCAATTGCCGGAAATACT	45.0	45.0	57.4	58.3	948-1171 bp	1.0	78.7