Hotspot

ndhC-trnV-UAC

LSC · 55881-56493 · 613 bp

Back to Engelhardia

Pi 0.019

MarkerSeek score 54.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 19
pi: 0.019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	34.8	50.0	56.1	57.8	629-768 bp	1.0	46.2
ndhC-trnV-UAC_p2	TGCCCAGAAAATATCATATTCGT	GAAGGTCTACGGTTCGAGTC	34.8	55.0	56.1	57.5	628-767 bp	1.0	46.2
ndhC-trnV-UAC_p3	TGCCCAGAAAATATCATATTCGT	GTTCGAGTCCGTATAGCCCT	34.8	55.0	56.1	58.7	617-756 bp	1.0	46.2
ndhC-trnV-UAC_p4	ATGCCCAGAAAATATCATATTCGT	AGAAGGTCTACGGTTCGAGT	33.3	50.0	56.6	57.8	630-769 bp	1.0	46.2
ndhC-trnV-UAC_p5	TGCCCAGAAAATATCATATTCGT	AAGGTCTACGGTTCGAGTCC	34.8	55.0	56.1	58.8	627-766 bp	1.0	46.2