Hotspot

psbZ-trnG-GCC

LSC · 40585-41001 · 417 bp

Back to Engelhardia

Pi 0.0112

MarkerSeek score 50.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 9
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	AGTCTTTCTGGTGGGTATCCT	ATTGAACCCGCATCTTCTCC	47.6	50.0	57.8	58.0	455-738 bp	1.0	75.1
psbZ-trnG-GCC_p2	AGTCTTTCTGGTGGGTATCCT	GGATAGCGGGAATTGAACCC	47.6	55.0	57.8	58.4	466-749 bp	1.0	72.3
psbZ-trnG-GCC_p3	AGTCTTTCTGGTGGGTATCCT	GGGAATTGAACCCGCATCTT	47.6	50.0	57.8	58.5	459-742 bp	1.0	71.3
psbZ-trnG-GCC_p4	AGTCTTTCTGGTGGGTATCC	ATTGAACCCGCATCTTCTCC	50.0	50.0	56.2	58.0	455-738 bp	1.0	70.6
psbZ-trnG-GCC_p5	GTCTTTCTGGTGGGTATCCT	ATTGAACCCGCATCTTCTCC	50.0	50.0	56.2	58.0	454-737 bp	1.0	70.6