Hotspot

trnS-GCU-trnG-UCC

LSC · 9359-10232 · 874 bp

Back to Engelhardia

Pi 0.00992
MarkerSeek score 57.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
874
variable_sites
16
pi
0.00992
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.92
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 893-943 bp 1.0 57.1
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 892-942 bp 1.0 57.1
trnS-GCU-trnG-UCC_p3 ACAAAAGCGGAAAGAGAGGG ACGAATCACACTTTTACCACT 50.0 38.1 58.1 55.5 953-1003 bp 1.0 56.2
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 896-946 bp 1.0 56.0
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 895-945 bp 1.0 56.0