Hotspot

trnK-UUU-rps16

LSC · 4229-5249 · 1021 bp

Back to Engelhardia

Pi 0.0274

MarkerSeek score 64.30

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 52
pi: 0.0274
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCAGTCGCGGTCTTACAAAG	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	57.9	57.6	1201-1238 bp	1.0	81.3
trnK-UUU-rps16_p2	TCAGTCGCGGTCTTACAAAG	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	57.9	57.6	1202-1239 bp	1.0	81.3
trnK-UUU-rps16_p3	TCAGTCGCGGTCTTACAAAG	TTTCAAAGAAGGCGGGAGTT	50.0	45.0	57.9	57.6	1203-1240 bp	1.0	81.3
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	58.2	57.6	1114-1151 bp	1.0	80.8
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	58.2	57.6	1115-1152 bp	1.0	80.8