Hotspot

ndhD

SSC · 121197-122702 · 1506 bp

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Pi 0.000664

MarkerSeek score 38.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 2
pi: 0.000664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGCATTGTACAGTGAACGA	ACCGACAAAAACCCGTACTC	40.0	50.0	55.6	58.1	1655 bp	1.0	60.1
ndhD_p2	TTGCATTGTACAGTGAACGA	GCGGGTTTTTCTGGTCAAAG	40.0	50.0	55.6	57.9	1618 bp	1.0	60.1
ndhD_p3	CATTGCATTGTACAGTGAACGA	ACCGACAAAAACCCGTACTC	40.9	50.0	57.6	58.1	1657 bp	1.0	59.8
ndhD_p4	TCATTGCATTGTACAGTGAACG	ACCGACAAAAACCCGTACTC	40.9	50.0	57.6	58.1	1658 bp	1.0	59.8
ndhD_p5	CATTGCATTGTACAGTGAACGA	GCGGGTTTTTCTGGTCAAAG	40.9	50.0	57.6	57.9	1620 bp	1.0	59.8