Hotspot

matK

LSC · 3676-5199 · 1524 bp

Back to Endiandra

Pi 0.00164

MarkerSeek score 59.80

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 5
pi: 0.00164
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CCACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	52.4	50.0	58.5	58.0	1715 bp	1.0	68.5
matK_p2	CCACTACACTACTGCATGAGC	TTGTTCTGACCGTATCGCAC	52.4	50.0	58.5	58.3	1714 bp	1.0	66.2
matK_p3	TCAACCACTACACTACTGCA	CTTGTTCTGACCGTATCGCA	45.0	50.0	56.1	58.0	1719 bp	1.0	65.2
matK_p4	ACCACTACACTACTGCATGAG	CTTGTTCTGACCGTATCGCA	47.6	50.0	57.1	58.0	1716 bp	1.0	65.1
matK_p5	CACTACACTACTGCATGAGC	CTTGTTCTGACCGTATCGCA	50.0	50.0	56.0	58.0	1714 bp	1.0	63.9