Hotspot

trnV-GAC-rps12

IRa · 143826-145613 · 1788 bp

Back to Endiandra

Pi 0.000559
MarkerSeek score 58.80
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1788
variable_sites
2
pi
0.000559
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 CGGGGGAGCAAGTCAAAATA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.9 57.7 1945-1950 bp 1.0 77.2
trnV-GAC-rps12_p2 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1869-1874 bp 1.0 75.4
trnV-GAC-rps12_p3 GGCTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.4 57.7 1894-1899 bp 1.0 75.4
trnV-GAC-rps12_p4 GCTCGAACTGATGACTTCCA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.6 57.7 1893-1898 bp 1.0 75.2
trnV-GAC-rps12_p5 CAGGCTCGAACTGATGACTT TGGCAACTTTACGTAAGGCA 50.0 45.0 57.6 57.7 1896-1901 bp 1.0 75.2