Hotspot

ndhG-ndhI

SSC · 124423-124815 · 393 bp

Back to Endiandra

Pi 0.00382

MarkerSeek score 58.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 393
variable_sites: 3
pi: 0.00382
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GTAATACCACCCCCAGACCT	TCAAATTGCTTTGGGTCGAT	55.0	40.0	58.1	55.9	545-550 bp	1.0	63.7
ndhG-ndhI_p2	AGTAATACCACCCCCAGACC	ATCAAATTGCTTTGGGTCGA	55.0	40.0	58.1	55.9	547-552 bp	1.0	63.6
ndhG-ndhI_p3	AGTAATACCACCCCCAGACC	TCAAATTGCTTTGGGTCGAT	55.0	40.0	58.1	55.9	546-551 bp	1.0	63.6
ndhG-ndhI_p4	GTAATACCACCCCCAGACCT	ATCAAATTGCTTTGGGTCGA	55.0	40.0	58.1	55.9	546-551 bp	1.0	63.6
ndhG-ndhI_p5	TAATACCACCCCCAGACCTC	TCAAATTGCTTTGGGTCGAT	55.0	40.0	57.8	55.9	544-549 bp	1.0	63.1