Hotspot

ndhF-rpl32

SSC · 117101-118291 · 1191 bp

Back to Endiandra

Pi 0.00351

MarkerSeek score 61.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1191
variable_sites: 8
pi: 0.00351
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAGCAAAAGGT	TGACATCCCTTTCCCTTCCA	50.0	50.0	58.0	58.2	1313-1322 bp	1.0	78.8
ndhF-rpl32_p2	CGGAACAAACAGAAGTCCCA	TGACATCCCTTTCCCTTCCA	50.0	50.0	58.0	58.2	1351-1360 bp	1.0	78.6
ndhF-rpl32_p3	CTGGAAGTGGAGCAAAAGGT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1259-1268 bp	1.0	78.4
ndhF-rpl32_p4	CGGAACAAACAGAAGTCCCA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1297-1306 bp	1.0	78.2
ndhF-rpl32_p5	CTGGAAGTGGAGCAAAAGGT	CTGACATCCCTTTCCCTTCC	50.0	55.0	58.0	57.6	1314-1323 bp	1.0	77.4