Hotspot

rps12-trnV-GAC

IRb · 102316-104103 · 1788 bp

Back to Endiandra

Pi 0.000559

MarkerSeek score 58.80

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1788
variable_sites: 2
pi: 0.000559
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	CGGGGGAGCAAGTCAAAATA	45.0	50.0	57.7	57.9	1945-1950 bp	1.0	77.2
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	45.0	55.0	57.7	57.7	1869-1874 bp	1.0	75.4
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	GGCTCGAACTGATGACTTCC	45.0	55.0	57.7	58.4	1894-1899 bp	1.0	75.4
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	GCTCGAACTGATGACTTCCA	45.0	50.0	57.7	57.6	1893-1898 bp	1.0	75.2
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	CAGGCTCGAACTGATGACTT	45.0	50.0	57.7	57.6	1896-1901 bp	1.0	75.2