Hotspot

clpP-psbB

LSC · 77085-77522 · 438 bp

Back to Endiandra

Pi 0.00457

MarkerSeek score 58.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 4
pi: 0.00457
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTTTTGGAACACCAATGGGC	GCCGACCAGGATCATTCAAT	50.0	50.0	57.8	58.0	515 bp	1.0	79.1
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	ATATGGACAGAAAGCAGCCG	50.0	50.0	57.8	58.0	531 bp	1.0	78.9
clpP-psbB_p3	ACCCAAACTGCATCTTCCTC	GCCGACCAGGATCATTCAAT	50.0	50.0	57.8	58.0	555 bp	1.0	78.7
clpP-psbB_p4	ACCCAAACTGCATCTTCCTC	ATATGGACAGAAAGCAGCCG	50.0	50.0	57.8	58.0	571 bp	1.0	78.6
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	562 bp	1.0	78.3