Hotspot

trnT-GGU-psbD

LSC · 35039-36426 · 1388 bp

Back to Endiandra

Pi 0.00144
MarkerSeek score 59.80
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1388
variable_sites
4
pi
0.00144
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTCCTTAACC 55.0 55.0 58.1 57.9 1530 bp 1.0 78.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTCCTTAACC 50.0 55.0 58.1 57.9 1533 bp 1.0 78.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1538 bp 1.0 77.7
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1541 bp 1.0 77.5
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CGAAACGGTCCCTCCTTAAC 55.0 55.0 58.1 58.3 1531 bp 1.0 77.4