Hotspot

atpA-atpF

LSC · 13693-13765 · 73 bp

Back to Endiandra

Pi 0.0399

MarkerSeek score 82.20

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 73
variable_sites: 5
pi: 0.0399
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCCACTTGAAGGACTGTTCC	CTGGGGGCCATGAAAGAAAT	55.0	50.0	58.1	58.1	207-210 bp	1.0	81.2
atpA-atpF_p2	CCCACTTGAAGGACTGTTCC	AAGGAGCCCTAGGAACTCTG	55.0	55.0	58.1	58.1	284-287 bp	1.0	80.4
atpA-atpF_p3	CCCACTTGAAGGACTGTTCC	CAAGGAGCCCTAGGAACTCT	55.0	55.0	58.1	58.1	285-288 bp	1.0	80.4
atpA-atpF_p4	CCCACTTGAAGGACTGTTCC	GGAGCCCTAGGAACTCTGAA	55.0	55.0	58.1	58.1	282-285 bp	1.0	80.4
atpA-atpF_p5	CCCACTTGAAGGACTGTTCC	TAATATTGGCATGCTGGGGG	55.0	50.0	58.1	57.7	220-223 bp	1.0	79.6