Hotspot

ycf1

SSC · 122240-127273 · 5034 bp

Back to Emilia

Pi 0.374
MarkerSeek score 72.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5034
variable_sites
1476
pi
0.374
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCGAGGAGGACCAAAAGGAT TATTTGGCGACCGTCTCAAG 50.0 50.0 58.0 58.0 604 bp 0.5 59.6
ycf1_p2 TCGAGGAGGACCAAAAGGAT AGAACCGGATTTTCGTCGAG 50.0 50.0 58.0 58.0 667 bp 0.5 59.6
ycf1_p3 TCGAGGAGGACCAAAAGGAT GACCGTCTCAAGGAAATCCC 50.0 55.0 58.0 58.0 596 bp 0.5 59.4
ycf1_p4 TCGAGGAGGACCAAAAGGAT TAATGCAAGGGCTCGATCAC 50.0 50.0 58.0 58.0 821 bp 0.5 59.2
ycf1_p5 TCGAGGAGGACCAAAAGGAT TTAACTCGTTCCAGCCGAAG 50.0 50.0 58.0 57.9 765 bp 0.5 59.0