Hotspot

ndhH

SSC · 120494-121672 · 1179 bp

Pi 0.454

MarkerSeek score 79.60

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1179
variable_sites: 432
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTTGTTGTATACCCGCAGA	CGCTTGCTGGCTTATTTGTC	50.0	50.0	58.0	58.1	1543 bp	0.5	59.2
ndhH_p2	CTGCAAATTTCGAAGCCGTT	CGCTTGCTGGCTTATTTGTC	45.0	50.0	57.9	58.1	2074 bp	0.5	58.8
ndhH_p3	CCAAAGGTCCTGCGTATTCA	CGCTTGCTGGCTTATTTGTC	50.0	50.0	57.9	58.1	1572 bp	0.5	58.3
ndhH_p4	CGTTGTTGTATACCCGCAGA	AATAATGGGAGCCGTTTGGG	50.0	50.0	58.0	58.2	1977 bp	0.5	58.2
ndhH_p5	ACCATCTGCTAGAGCTTGGA	CGCTTGCTGGCTTATTTGTC	50.0	50.0	58.1	58.1	1598 bp	0.5	58.2