Hotspot

ccsA

SSC · 112830-113801 · 972 bp

Pi 0.452

MarkerSeek score 82.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 394
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1578 bp	0.5	59.6
ccsA_p2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1577 bp	0.5	59.6
ccsA_p3	GTGTGGTGGGGAGTCTTTTT	GTTTTTGATTCTGGACCGCG	50.0	50.0	57.9	58.0	1704 bp	0.5	59.2
ccsA_p4	GTGAAATCGGTAGACACGCT	GCATTACCGGGTATGAGTGG	50.0	55.0	58.0	58.1	1779 bp	0.5	59.0
ccsA_p5	TGAAATCGGTAGACACGCTG	GCATTACCGGGTATGAGTGG	50.0	55.0	58.0	58.1	1778 bp	0.5	59.0