Hotspot

ndhH-rps15

SSC · 121673-121763 · 91 bp

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Pi 0.412
MarkerSeek score 76.90
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
91
variable_sites
35
pi
0.412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 TGAGGTAAATCAGCAGCGAC CGCTTGCTGGCTTATTTGTC 50.0 50.0 58.0 58.1 684 bp 0.5 59.1
ndhH-rps15_p2 CAGAAGCTCGTAGCATTGGT CGCTTGCTGGCTTATTTGTC 50.0 50.0 58.0 58.1 860 bp 0.5 59.1
ndhH-rps15_p3 AACACCATGCATTGATGGGT CGCTTGCTGGCTTATTTGTC 45.0 50.0 58.1 58.1 253 bp 0.5 58.7
ndhH-rps15_p4 CCTAATTGCTCTGGGCCATT CGCTTGCTGGCTTATTTGTC 50.0 50.0 57.9 58.1 447 bp 0.5 58.6
ndhH-rps15_p5 TGGGATTCCGCGTAATAAGC CGCTTGCTGGCTTATTTGTC 50.0 50.0 58.1 58.1 770 bp 0.5 58.2