Hotspot

ndhE-ndhG

SSC · 116503-116734 · 232 bp

Back to Emilia

Pi 0.477
MarkerSeek score 76.90
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
232
variable_sites
103
pi
0.477
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 TTTGGCTTTACATCCGTCCC TGGAGTAACTTCCGTGGTCT 50.0 50.0 58.2 58.0 1138 bp 0.5 58.6
ndhE-ndhG_p2 CTTCTGGATTGACAGTGCCA TGGAGTAACTTCCGTGGTCT 50.0 50.0 57.8 58.0 907 bp 0.5 58.4
ndhE-ndhG_p3 ACTTCTGGATTGACAGTGCC TGGAGTAACTTCCGTGGTCT 50.0 50.0 57.8 58.0 908 bp 0.5 58.4
ndhE-ndhG_p4 ACATCCGTCCCATGGTATCA TGGAGTAACTTCCGTGGTCT 50.0 50.0 58.2 58.0 1129 bp 0.5 58.4
ndhE-ndhG_p5 GGACACATTGAGTACACCCG TGGAGTAACTTCCGTGGTCT 55.0 50.0 58.3 58.0 1082 bp 0.5 57.7