Hotspot

rps16

LSC · 5476-6643 · 1168 bp

Back to Emblica

Pi 0.0366

MarkerSeek score 71.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1168
variable_sites: 42
pi: 0.0366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGTTATAAATTATCCACACCCTCT	ACCGGTTCAAAATTCAAGGA	36.0	40.0	57.5	55.5	1231 bp	0.5	20.0
rps16_p2	TGGTTATAAATTATCCACACCCTCT	ACCGGTTCAAAATTCAAGGAA	36.0	38.1	57.5	56.3	1231 bp	0.5	20.0
rps16_p3	TGGTTATAAATTATCCACACCCTC	ACCGGTTCAAAATTCAAGGA	37.5	40.0	56.2	55.5	1231 bp	0.5	20.0
rps16_p4	TGGTTATAAATTATCCACACCCT	ACCGGTTCAAAATTCAAGGA	34.8	40.0	55.1	55.5	1231 bp	0.5	20.0
rps16_p5	TGGTTATAAATTATCCACACCCTC	ACCGGTTCAAAATTCAAGGAA	37.5	38.1	56.2	56.3	1231 bp	0.5	20.0