Hotspot

ndhA

SSC · 120979-123219 · 2241 bp

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Pi 0.0318

MarkerSeek score 62.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 70
pi: 0.0318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GCAGCTCGTATCGTTTGTTG	AGAGTTGAAGCACCAAAGGG	50.0	50.0	57.9	58.0	2549-2592 bp	1.0	82.7
ndhA_p2	GCAGCTCGTATCGTTTGTTG	AACCCGCTGCTTTGATAGAG	50.0	50.0	57.9	58.0	2661-2704 bp	1.0	82.4
ndhA_p3	ACTCAACACAGTTGCCACAA	AGAGTTGAAGCACCAAAGGG	45.0	50.0	58.2	58.0	2830-2873 bp	1.0	82.4
ndhA_p4	CGTGACGATCATACGTGGAA	AGAGTTGAAGCACCAAAGGG	50.0	50.0	57.8	58.0	2893-2936 bp	1.0	82.2
ndhA_p5	TTCGTGACGATCATACGTGG	AGAGTTGAAGCACCAAAGGG	50.0	50.0	57.8	58.0	2938 bp	0.5	58.4