Hotspot

rps15-ycf1

SSC · 124783-125173 · 391 bp

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Pi 0.0472

MarkerSeek score 81.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 391
variable_sites: 18
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGG	45.0	50.0	57.5	56.8	526-528 bp	1.0	71.0
rps15-ycf1_p2	TGAAATTCAACCGATCCCGT	GCTAGTCGGTTCAGTATGGT	45.0	50.0	57.5	56.7	525-527 bp	1.0	71.0
rps15-ycf1_p3	TGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGGT	45.0	47.6	57.5	58.5	526-528 bp	1.0	68.6
rps15-ycf1_p4	TGAAATTCAACCGATCCCGT	ATGCTAGTCGGTTCAGTATGG	45.0	47.6	57.5	57.3	527-529 bp	1.0	67.1
rps15-ycf1_p5	TTGAAATTCAACCGATCCCGT	TGCTAGTCGGTTCAGTATGG	42.9	50.0	58.2	56.8	527-529 bp	1.0	65.1