Hotspot

ndhF-rpl32

SSC · 113544-114486 · 943 bp

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Pi 0.0574

MarkerSeek score 74.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 943
variable_sites: 41
pi: 0.0574
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGCGTAATTAAAGGTAAAATCCATGA	AGCTTTCAACGCTACCCAAT	30.8	45.0	57.5	57.8	874-1083 bp	1.0	47.4
ndhF-rpl32_p2	AGCGTAATTAAAGGTAAAATCCATGA	AAAGCTTTCAACGCTACCCA	30.8	45.0	57.5	58.0	876-1085 bp	1.0	47.3
ndhF-rpl32_p3	AGCGTAATTAAAGGTAAAATCCATGA	AAGCTTTCAACGCTACCCAA	30.8	45.0	57.5	58.0	875-1084 bp	1.0	47.3
ndhF-rpl32_p4	AGCGTAATTAAAGGTAAAATCCA	AAAGCTTTCAACGCTACCCA	30.4	45.0	54.3	58.0	876-1085 bp	1.0	47.3
ndhF-rpl32_p5	AGCGTAATTAAAGGTAAAATCCA	AAGCTTTCAACGCTACCCAA	30.4	45.0	54.3	58.0	875-1084 bp	1.0	47.3