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petA-psbJ

LSC · 64809-65721 · 913 bp

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Pi 0.0627

MarkerSeek score 81.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 913
variable_sites: 56
pi: 0.0627
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCGAGAAAGTTCAATTGTCCGA	ACTGGTATTCCTGTGATCGG	40.9	50.0	58.0	56.7	1023-1206 bp	1.0	59.1
petA-psbJ_p2	TCGAGAAAGTTCAATTGTCCGA	CTGGTATTCCTGTGATCGGT	40.9	50.0	58.0	56.7	1022-1205 bp	1.0	59.1
petA-psbJ_p3	TCGAGAAAGTTCAATTGTCCGA	ACTGGTATTCCTGTGATCGGT	40.9	47.6	58.0	58.5	1023-1206 bp	1.0	57.1
petA-psbJ_p4	TCGAGAAAGTTCAATTGTCCGA	AACTGGTATTCCTGTGATCGG	40.9	47.6	58.0	57.5	1024-1207 bp	1.0	57.0
petA-psbJ_p5	TCGAGAAAGTTCAATTGTCCGA	CTGGTATTCCTGTGATCGGTT	40.9	47.6	58.0	57.5	1022-1205 bp	1.0	57.0