Hotspot

trnF-GAA-ndhJ

LSC · 49470-49974 · 505 bp

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Pi 0.0483

MarkerSeek score 76.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 24
pi: 0.0483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	652-653 bp	1.0	83.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	651-652 bp	1.0	83.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	616-617 bp	1.0	83.4
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	615-616 bp	1.0	83.4
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.9	58.0	639-640 bp	1.0	82.6