Hotspot

atpH-atpI

LSC · 14245-15374 · 1130 bp

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Pi 0.0365

MarkerSeek score 74.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 40
pi: 0.0365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1184-1217 bp	1.0	83.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	45.0	55.0	58.0	58.2	1158-1191 bp	1.0	83.2
atpH-atpI_p3	CAGTACCTTGACCAACTCCG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1243-1276 bp	1.0	82.9
atpH-atpI_p4	CAGTACCTTGACCAACTCCG	TAGGTGAATCCATGGAGGGG	55.0	55.0	57.9	58.2	1217-1250 bp	1.0	82.7
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1222-1255 bp	1.0	82.6