Hotspot

ycf1

SSC · 126802-132396 · 5595 bp

Back to Embelia

Pi 0.00955

MarkerSeek score 45.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 93
pi: 0.00955
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTCAGTCTCCATTCCCCA	TGGTGTTCAATTATCAAAAAGAGA	50.0	29.2	58.0	55.0	195 bp	1.0	42.9
ycf1_p2	AGTTCAGTCTCCATTCCCCA	TGGTGTTCAATTATCAAAAAGAGAA	50.0	28.0	58.0	55.7	195 bp	1.0	42.9
ycf1_p3	TTTCGGGGAGAACCAAAAGG	TGGTGTTCAATTATCAAAAAGAGA	50.0	29.2	58.0	55.0	216 bp	1.0	42.6
ycf1_p4	TTTCGGGGAGAACCAAAAGG	TGGTGTTCAATTATCAAAAAGAGAA	50.0	28.0	58.0	55.7	216 bp	1.0	42.6
ycf1_p5	TTTCGGGGAGAACCAAAAGG	TGGTGTTCAATTATCAAAAAGAG	50.0	30.4	58.0	53.5	216 bp	1.0	42.6