Hotspot

clpP-psbB

LSC · 74500-74953 · 454 bp

Back to Embelia

Pi 0.0327

MarkerSeek score 69.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 454
variable_sites: 25
pi: 0.0327
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCCAGGACTCCGAAAAGGTA	GACCGGGATCATTCAACACA	50.0	50.0	57.7	57.9	548-554 bp	1.0	80.3
clpP-psbB_p2	CCAGGACTCCGAAAAGGTAC	GACCGGGATCATTCAACACA	55.0	50.0	57.6	57.9	547-553 bp	1.0	79.7
clpP-psbB_p3	TCCAGGACTCCGAAAAGGTA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	516-522 bp	1.0	79.5
clpP-psbB_p4	CCAGGACTCCGAAAAGGTAC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.6	58.2	515-521 bp	1.0	78.9
clpP-psbB_p5	GATGCATCTTCCTCTCCAGG	GACCGGGATCATTCAACACA	55.0	50.0	57.5	57.9	562-568 bp	1.0	78.3