Hotspot

psaJ-rpl33

LSC · 69468-70000 · 533 bp

Back to Embelia

Pi 0.0254

MarkerSeek score 55.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 21
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATGCGTTGACATTCCCCTTT	CTCGGACATCTTTCCCCTTG	45.0	55.0	58.1	58.0	567-592 bp	1.0	86.2
psaJ-rpl33_p2	TGTGGCACCGGTACTAAGTA	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	644-669 bp	1.0	85.6
psaJ-rpl33_p3	ACCCGTGACATTTTGTACCC	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1118-1155 bp	1.0	84.2
psaJ-rpl33_p4	CCCGTGACATTTTGTACCCA	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1117-1154 bp	1.0	84.2
psaJ-rpl33_p5	ACAGGATTTGAACCCGTGAC	CTCGGACATCTTTCCCCTTG	50.0	55.0	58.1	58.0	1129-1166 bp	1.0	84.1