Hotspot

petA-psbJ

LSC · 65067-66117 · 1051 bp

Back to Embelia

Pi 0.0188

MarkerSeek score 67.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 34
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	GCCGATACTACTGGAAGGAT	45.0	50.0	57.8	56.2	1239-1255 bp	1.0	67.6
petA-psbJ_p2	GCATCTGTTGTTTTGGCACA	GCCGATACTACTGGAAGGATT	45.0	47.6	57.8	57.0	1239-1255 bp	1.0	65.7
petA-psbJ_p3	ATTACGCGTTCAAGGCCTTT	GCCGATACTACTGGAAGGAT	45.0	50.0	58.5	56.2	1270-1286 bp	1.0	65.5
petA-psbJ_p4	GCATCTGTTGTTTTGGCACA	GCCGATACTACTGGAAGGATTC	45.0	50.0	57.8	58.1	1239-1255 bp	1.0	65.3
petA-psbJ_p5	AGCATCTGTTGTTTTGGCAC	GCCGATACTACTGGAAGGAT	45.0	50.0	57.5	56.2	1240-1256 bp	1.0	65.3