Hotspot

ndhC-trnV-UAC

LSC · 52057-53200 · 1144 bp

Back to Embelia

Pi 0.0264

MarkerSeek score 79.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1144
variable_sites: 54
pi: 0.0264
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGCTAATCGGGGCTAAAAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1265-1284 bp	1.0	83.3
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1257-1276 bp	1.0	82.1
ndhC-trnV-UAC_p3	CTCTGGTCCTTGGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	1275-1294 bp	1.0	81.7
ndhC-trnV-UAC_p4	CTTGGCTAATCGGGGCTAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1267-1286 bp	1.0	80.9
ndhC-trnV-UAC_p5	GGCTAATCGGGGCTAAAACT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1264-1283 bp	1.0	80.9