Hotspot

rps4-trnT-UGU

LSC · 47517-47865 · 349 bp

Back to Embelia

Pi 0.0239

MarkerSeek score 66.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 15
pi: 0.0239
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AAGATCATTTCCGGCTCTGG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.7	57.9	493-508 bp	1.0	82.6
rps4-trnT-UGU_p2	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	462-477 bp	1.0	82.2
rps4-trnT-UGU_p3	AAGATCATTTCCGGCTCTGG	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.7	57.8	461-476 bp	1.0	81.9
rps4-trnT-UGU_p4	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	430-445 bp	1.0	81.5
rps4-trnT-UGU_p5	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	461-476 bp	1.0	79.8