Hotspot

petN-psbM

LSC · 29200-30121 · 922 bp

Back to Embelia

Pi 0.0219

MarkerSeek score 73.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 922
variable_sites: 39
pi: 0.0219
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCTCGTGCCTACTGCTTTTC	50.0	50.0	58.2	57.9	1067-1141 bp	1.0	85.0
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CTCGTGCCTACTGCTTTTCT	50.0	50.0	58.2	57.9	1066-1140 bp	1.0	84.9
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACTGCATTGTTCGTTCTCGT	50.0	45.0	58.2	58.1	1081-1155 bp	1.0	84.5
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	GTTCTCGTGCCTACTGCTTT	50.0	50.0	58.2	58.2	1069-1143 bp	1.0	84.0
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	CTGCATTGTTCGTTCTCGTG	50.0	50.0	58.2	57.8	1080-1154 bp	1.0	83.8