Hotspot

trnR-UCU-atpA

LSC · 10921-11048 · 128 bp

Back to Embelia

Pi 0.0286

MarkerSeek score 65.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 128
variable_sites: 6
pi: 0.0286
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	CGGAATGAATGAAAAGCGTCC	CAGGAACAGATGGAACGCTT	47.6	50.0	58.2	58.2	256-257 bp	1.0	71.3
trnR-UCU-atpA_p2	CGGAATGAATGAAAAGCGTCC	TTCAGGAACAGATGGAACGC	47.6	50.0	58.2	58.2	258-259 bp	1.0	71.2
trnR-UCU-atpA_p3	CCAAACAAGAGGCAAAATCGG	CAGGAACAGATGGAACGCTT	47.6	50.0	58.3	58.2	274-275 bp	1.0	70.2
trnR-UCU-atpA_p4	CCAAACAAGAGGCAAAATCGG	TTCAGGAACAGATGGAACGC	47.6	50.0	58.3	58.2	276-277 bp	1.0	70.2
trnR-UCU-atpA_p5	CGGAATGAATGAAAAGCGTCC	AGGAACAGATGGAACGCTTT	47.6	45.0	58.2	57.4	255-256 bp	1.0	68.2