Hotspot

rps16-trnQ-UUG

LSC · 6014-7776 · 1763 bp

Back to Embelia

Pi 0.0177

MarkerSeek score 63.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1763
variable_sites: 52
pi: 0.0177
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCATGTCTTTCAAGCCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.2	58.1	1366-1865 bp	1.0	84.4
rps16-trnQ-UUG_p2	GATCATGTCTTTCAAGCCGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	1367-1866 bp	1.0	82.1
rps16-trnQ-UUG_p3	ATCATGTCTTTCAAGCCGCA	AAATTTAAATGGGGCGTGGC	45.0	45.0	58.2	57.6	1423-1922 bp	1.0	81.8
rps16-trnQ-UUG_p4	ATCATGTCTTTCAAGCCGCA	GGGTTTTGGTCCCGGTATTC	45.0	55.0	58.2	58.5	1387-1886 bp	1.0	80.7
rps16-trnQ-UUG_p5	ATCATGTCTTTCAAGCCGCA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.2	57.3	1370-1869 bp	1.0	79.7