Hotspot

trnH-GUG-psbA

LSC · 76-492 · 417 bp

Back to Embelia

Pi 0.0311

MarkerSeek score 67.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 24
pi: 0.0311
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CCACTTGGCTACATCCGC	TTCCCTCTAGACCTAGCTGC	61.1	55.0	58.2	57.9	483-489 bp	1.0	69.3
trnH-GUG-psbA_p2	CCACTTGGCTACATCCGC	TAGACCTAGCTGCGGTTGAA	61.1	50.0	58.2	58.4	476-482 bp	1.0	66.4
trnH-GUG-psbA_p3	CCACTTGGCTACATCCGC	CTCTAGACCTAGCTGCGGTT	61.1	55.0	58.2	58.6	479-485 bp	1.0	65.1
trnH-GUG-psbA_p4	CCACTTGGCTACATCCGC	CGGTTGAAGCTCCATCTACA	61.1	50.0	58.2	57.3	464-470 bp	1.0	64.8
trnH-GUG-psbA_p5	CCACTTGGCTACATCCGC	GCGGTTGAAGCTCCATCTAC	61.1	55.0	58.2	58.7	465-471 bp	1.0	64.5