Hotspot

psbB

LSC · 69600-71126 · 1527 bp

Back to Elytrophorus

Pi 0.00458

MarkerSeek score 61.30

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 7
pi: 0.00458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCTGACACAGAATGGTTAGGT	AGGACAGGAGATGTTTCCCA	42.9	50.0	56.3	58.0	1677 bp	1.0	58.3
psbB_p2	TTCTGACACAGAATGGTTAGGT	AGGACAGGAGATGTTTCCCA	40.9	50.0	57.0	58.0	1678 bp	1.0	56.2
psbB_p3	TCATGCTTTCTGACACAGAA	AGGACAGGAGATGTTTCCCA	40.0	50.0	54.9	58.0	1685 bp	1.0	55.3
psbB_p4	TTCATGCTTTCTGACACAGA	AGGACAGGAGATGTTTCCCA	40.0	50.0	54.9	58.0	1686 bp	1.0	55.3
psbB_p5	CTGACACAGAATGGTTAGGTACA	AGGACAGGAGATGTTTCCCA	43.5	50.0	57.9	58.0	1676 bp	1.0	54.8